女黄颗粒对免疫抑制模型小鼠脾组织病理及细胞周期的影响

doi:10.11843/j.issn.0366-6964.2018.12.027

Abstract

Abstract:

This study was conducted to analyze the effects of Nühuang granule on histopathology and cell cycle in spleen of immunosuppressed mice. Fifty healthy Kunming mice were randomly divided into blank group, model group, and high-, middle-, and low-dose Nühuang granule groups, with 10 rats in each group. All groups were treated with intraperitoneal injection of cyclophosphamide (80 mg·kg^-1, once a day and for 5 days) to replicate the immunosuppressive model except for the blank group. After model replicated successfully, each group was gavaged with normal saline or drugs for 7 days consecutively. After injection of cyclophosphamide, the spleen index, the number of blood leukocytes, the percentage of lymphocytes and monocytes of mice were decreased. Spleen tissues were damaged and the percentage of G0/G1 phase cells was reduced, and the cells in S phase was increased significantly. While the spleen index, number of blood leukocyte, percentage of lymphocyte and mononuclear of mice were increased, spleen tissue structure was improved, the percentage of spleen cells in G0/G1 phase was increased significantly, and the cells in S phase decreased after Nühuang granule was gavaged to the test mice. Among them, the effect of intermediate dose group was best. Nühuang granule can improve the spleen tissue structure in the immunosuppressed mice. It also can increase the percentage of G0/G1 phase cells, decrease the percentage of S phase cells, and increase the spleen index of test mice.

CLC Number:

S853.9

YAN Zhi-qiang, ZHENG Hua, ZHU Mai-xun, FU Wen-gui, ZHANG Yi-fan, ZHAI Shao-qin. Effect of Nühuang Granules on Spleen Histopathology and Cell Cycle of Immunosuppressive Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2771-2776.

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Effect of Nühuang Granules on Spleen Histopathology and Cell Cycle of Immunosuppressive Mice

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